Structural model of human dUTPase in complex with a novel proteinaceous inhibitor.

Nyíri K, Mertens HDT, Tihanyi B, Nagy GN, Kőhegyi B, Matejka J, Harris MJ, Szabó JE, Papp-Kádár V, Németh-Pongrácz V, Ozohanics O, Vékey K, Svergun DI, Borysik AJ, Vértessy BG, Sci Rep 8(1):4326 (2018) Europe PMC

SASDC87 – Deoxyuridine 5'-triphosphate nucleotidohydrolase complex with SaPIbov1 pathogenicity island repressor [3:2] (SEC-SAXS)

Deoxyuridine 5'-triphosphate nucleotidohydrolase
SaPIbov1 pathogenicity island repressor
MWI(0) 121 kDa
MWexpected 118 kDa
VPorod 190 nm3
log I(s) 4.60×10-2 4.60×10-3 4.60×10-4 4.60×10-5
Deoxyuridine 5'-triphosphate nucleotidohydrolase SaPIbov1 pathogenicity island repressor small angle scattering data  s, nm-1
ln I(s)
Deoxyuridine 5'-triphosphate nucleotidohydrolase SaPIbov1 pathogenicity island repressor Guinier plot ln 4.60×10-2 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Deoxyuridine 5'-triphosphate nucleotidohydrolase SaPIbov1 pathogenicity island repressor Kratky plot 1.104 0 √3 sRg
p(r)
Deoxyuridine 5'-triphosphate nucleotidohydrolase SaPIbov1 pathogenicity island repressor pair distance distribution function Rg: 3.9 nm 0 Dmax: 14 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Deoxyuridine 5'-triphosphate nucleotidohydrolase SaPIbov1 pathogenicity island repressor DAMMIF model
log I(s)
 s, nm-1
Deoxyuridine 5'-triphosphate nucleotidohydrolase SaPIbov1 pathogenicity island repressor CORAL model
Synchrotron SAXS data from solutions of Deoxyuridine 5'-triphosphate nucleotidohydrolase complex with SaPIbov1 pathogenicity island repressor [3:2] (SEC-SAXS) in 50 mM HEPES 300 mM NaCl 5 mM MgCl2, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 10°C. 3600 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Concentration = UNKNOWN

Deoxyuridine 5'-triphosphate nucleotidohydrolase (hDUT)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Trimer
Mon. MW   18.0 kDa
 
UniProt   P33316 (94-252)
Sequence   FASTA
 
SaPIbov1 pathogenicity island repressor (Stl)
Mol. type   Protein
Organism   Staphylococcus aureus
Olig. state   Dimer
Mon. MW   32.0 kDa
 
UniProt   E2FZP6 (13-278)
Sequence   FASTA