Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes.

Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C, Kolbe M, J Mol Biol 431(19):3787-3803 (2019) Europe PMC

SASDC98 – Surface presentation of antigens protein SpaOc-SpaO N-terminus

Surface presentation of antigens protein SpaO SpaO(SPOA2)
Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus
MWexperimental 36 kDa
MWexpected 42 kDa
VPorod 68 nm3
log I(s) 4.75×103 4.75×102 4.75×101 4.75×100
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus small angle scattering data  s, nm-1
ln I(s)
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus Guinier plot ln 4.76×103 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus Kratky plot 1.104 0 √3 sRg
p(r)
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus pair distance distribution function Rg: 3.1 nm 0 Dmax: 10.6 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Surface presentation of antigens protein SpaOc-SpaO N-terminus in 20 mM HEPES pH 7.5, 150 mM NaCl, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 9.50 mg/ml was measured at 20°C. 3600 successive frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN

Surface presentation of antigens protein SpaO SpaO(SPOA2) (SpaOc)
Mol. type   Protein
Organism   Salmonella enterica subsp. enterica serovar Typhimurium
Olig. state   Dimer
Mon. MW   12.4 kDa
 
UniProt   P40699 (203-303)
Sequence   FASTA
 
Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus (SpaO N-terminus)
Mol. type   Protein
Organism   Salmonella enterica subsp. enterica serovar Typhimurium
Olig. state   Monomer
Mon. MW   17.4 kDa
 
UniProt   P40699 (1-145)
Sequence   FASTA