MWexperimental | 29 | kDa |
MWexpected | 29 | kDa |
VPorod | 48 | nm3 |
log I(s)
8.23×102
8.23×101
8.23×100
8.23×10-1
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s, nm-1
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Synchrotron SAXS
data from solutions of
Sensory box domain of the sensory-box/GGDEF protein SO_1695 from Shewanella oneidensis, Northeast Structural Genomics Consortium Target SoR288B
in
5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH 7.5
were collected
on the
BL4-2 beam line
at the Stanford Synchrotron Radiation Lightsource (SSRL) storage ring
(Menlo Park, CA, USA)
using a Rayonix MX225-HE detector
at a sample-detector distance of 1.5 m and
at a wavelength of λ = 0.13 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 3.9 and 12.3 mg/ml were measured
at 20°C.
20 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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