Small angle X-ray scattering as a complementary tool for high-throughput structural studies.

Grant TD, Luft JR, Wolfley JR, Tsuruta H, Martel A, Montelione GT, Snell EH, Biopolymers 95(8):517-30 (2011) Europe PMC

SASDCC6 – Sensory box domain of the sensory-box/GGDEF protein SO_1695 from Shewanella oneidensis, Northeast Structural Genomics Consortium Target SoR288B

Diguanylate cyclase with PAS sensory domain
MWexperimental 29 kDa
MWexpected 29 kDa
VPorod 48 nm3
log I(s) 8.23×102 8.23×101 8.23×100 8.23×10-1
Diguanylate cyclase with PAS sensory domain small angle scattering data  s, nm-1
ln I(s)
Diguanylate cyclase with PAS sensory domain Guinier plot ln 8.24×102 Rg: 2.0 nm 0 (2.0 nm)-2 s2
(sRg)2I(s)/I(0)
Diguanylate cyclase with PAS sensory domain Kratky plot 1.104 0 3 sRg
p(r)
Diguanylate cyclase with PAS sensory domain pair distance distribution function Rg: 2.0 nm 0 Dmax: 6.4 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Diguanylate cyclase with PAS sensory domain PDB (PROTEIN DATA BANK) model

log I(s)
 s, nm-1
Diguanylate cyclase with PAS sensory domain DAMFILT model

Synchrotron SAXS data from solutions of Sensory box domain of the sensory-box/GGDEF protein SO_1695 from Shewanella oneidensis, Northeast Structural Genomics Consortium Target SoR288B in 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH 7.5 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL) storage ring (Menlo Park, CA, USA) using a Rayonix MX225-HE detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.13 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 3.9 and 12.3 mg/ml were measured at 20°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Diguanylate cyclase with PAS sensory domain
Mol. type   Protein
Organism   Shewanella oneidensis
Olig. state   Dimer
Mon. MW   14.7 kDa
 
UniProt   Q8EGB0
Sequence   FASTA
 
PDB ID   3MFX