A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover.

Deyaert E, Wauters L, Guaitoli G, Konijnenberg A, Leemans M, Terheyden S, Petrovic A, Gallardo R, Nederveen-Schippers LM, Athanasopoulos PS, Pots H, Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W, Nat Commun 8(1):1008 (2017) Europe PMC

SASDCD2 – Rab family protein CtRoc-COR GppNHp

Rab family protein
MWexperimental 78 kDa
MWexpected 130 kDa
VPorod 154 nm3
log I(s) 6.92×10-2 6.92×10-3 6.92×10-4 6.92×10-5
Rab family protein small angle scattering data  s, nm-1
ln I(s)
Rab family protein Guinier plot ln 6.92×10-2 Rg: 3.4 nm 0 (3.4 nm)-2 s2
(sRg)2I(s)/I(0)
Rab family protein Kratky plot 1.104 0 √3 sRg
p(r)
Rab family protein pair distance distribution function Rg: 3.3 nm 0 Dmax: 10.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Rab family protein CtRoc-COR (GppNHp) in 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GppNHp, pH 7.5 were collected on the SWING camera on the storage ring SOLEIL (Saint-Aubin, France) using a CCD AVIEX detector at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). Inline SEC-SAXS was employed at 25°C (50 µl injection volume of an 8 mg/ml protein solution on a Bio SEC-3 HPLC column (Agilent, 3 µm 300 Å)). 31 successive 0.750 second frames were collected through the elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled and averaged to produce the final SAXS data displayed in this entry.

Cell temperature = UNKNOWN. Storage temperature = UNKNOWN

Rab family protein
Mol. type   Protein
Organism   Chlorobaculum tepidum
Olig. state   Dimer
Mon. MW   64.9 kDa
 
UniProt   Q8KC98
Sequence   FASTA