SASDCD8 – Horse spleen apoferritin (WAXS)

Horse spleen apoferritin

MW^experimental	540	kDa
MW^expected	476	kDa

log I(s) 5.11×10⁴ 5.11×10³ 5.11×10² 5.11×10¹

Horse spleen apoferritin small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 5.12×10⁴ R_g: 6.3 nm 0 (6.3 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

Data validation

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Horse spleen apoferritin PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of Horse spleen apoferritin in tbs, pH 7.5 were collected on the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany), using a Pilatus 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.062 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). A solute concentration of 15.8 mg/ml was measured at 20°C. 40 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data scaled for protein concentration.

Tags: benchmark

Horse spleen apoferritin (Apoferritin)
Mol. type		Protein
Organism		Equus caballus
Olig. state		24-mer
Mon. MW		19.8 kDa

UniProt		P02791
Sequence		FASTA

PDB ID		1IER