SASDCH8 – Horse heart cytochrome C (WAXS)

Cytochrome c

MW^experimental	7	kDa
MW^expected	12	kDa

log I(s) 1.16×10⁴ 1.16×10³ 1.16×10² 1.16×10¹

Cytochrome c small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 1.17×10⁴ R_g: 1.2 nm 0 (1.2 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

Data validation

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Cytochrome c PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of horse heart cytochrome C in tbs, pH 7.5 were collected on the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.062 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 2.60 mg/ml was measured at 20°C. 34 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the scaled to protein concentration.

Tags: benchmark

Cytochrome c
Mol. type		Protein
Organism		Equus caballus
Olig. state		Monomer
Mon. MW		11.7 kDa

UniProt		P00004
Sequence		FASTA

PDB ID		1HRC