| MWexperimental | 143 | kDa |
| MWexpected | 136 | kDa |
| VPorod | 271 | nm3 |
|
log I(s)
2.35×102
2.35×101
2.35×100
2.35×10-1
|
s, nm-1
|
The GDP-switched GAF domain of DcpA modulates the concerted synthesis/hydrolysis of c-di-GMP in Mycobacterium smegmatis.
Chen HJ, Li N, Luo Y, Jiang YL, Zhou CZ, Chen Y, Li Q, Biochem J 475(7):1295-1308 (2018) Europe PMCSASDCJ9 – Bifunctional enzyme responsible for the synthesis and hydrolysis of c-di-GMP (DcpA) without GDP
Sensory box/response regulator|
|
|
Fits and models
|
|
|
|
|
Synchrotron SAXS data from solutions of bifunctional enzyme responsible for the synthesis and hydrolysis of c-di-GMP (DcpA) without GDP in 20 mM HEPES, 100 mM NaCl, 5% glycerol, 2 mM MgCl2, pH 7.5, were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.3 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 20°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.
|
|
|||||||||||||||||||||||||||||||||