WAXS benchmark on standard proteins

Maxim Petoukhov.

SASDCL8 – Horse myoglobin (WAXS)

Myoglobin
MWexperimental 17 kDa
MWexpected 17 kDa
log I(s) 1.06×103 1.06×102 1.06×101 1.06×100
Myoglobin small angle scattering data  s, nm-1
ln I(s)
Myoglobin Guinier plot ln 1.07×103 Rg: 1.6 nm 0 (1.6 nm)-2 s2
(sRg)2I(s)/I(0)
Myoglobin Kratky plot 1.104 0 √3 sRg

Data validation

Fits and models

log I(s)
 s, nm-1
Myoglobin PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of horse myoglobin (WAXS) in 100 mM Tris, 100 mM NaCl, pH 7.5 were collected on the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.062 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 5.20 mg/ml was measured at 10°C. 54 successive 0.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data scaled to protein concentration.

Sample detector distance = UNKNOWN. Concentration min = UNKNOWN

Tags: benchmark
Myoglobin
Mol. type   Protein
Organism   Equus caballus
Olig. state   Monomer
Mon. MW   17.1 kDa
 
UniProt   P68082
Sequence   FASTA
 
PDB ID   1NPF