| MWexperimental | 279 | kDa |
| MWexpected | 227 | kDa |
| VPorod | 511 | nm3 |
|
log I(s)
4.70×102
4.70×101
4.70×100
4.70×10-1
|
s, nm-1
|
Signaling ammonium across membranes through an ammonium sensor histidine kinase.
Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA, Nat Commun 9(1):164 (2018) Europe PMCSASDCM9 – Ammonium transporter Ks-Amt5
ammonium sensor/transducer|
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Fits and models
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Synchrotron SAXS
data from solutions of
Ammonium transporter Ks-Amt5
in
20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, 0.09%(w/v) CYMAL-5, pH 8
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.124 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample
at 10 mg/ml was injected
onto a column
at 10°C.
3600 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
MW estimate from excluded volume (DAMMIN) |
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