| MWI(0) | 22 | kDa |
| MWexpected | 17 | kDa |
| VPorod | 25 | nm3 |
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log I(s)
2.70×10-2
2.70×10-3
2.70×10-4
2.70×10-5
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s, nm-1
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2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update.
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, Tainer JA, Vachette P, Westbrook J, Whitten AE, Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017) Europe PMCSASDCQ2 – 4Ca2+-calmodulin - Xenopus laevis
Calmodulin-1|
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Fits and models
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Rg, nm
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Synchrotron SAXS data from solutions of calcium-loaded calmodulin in 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH 7.5 were collected on the SAXS/WAXS beam line at Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.10332 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Scattering data are on an absolute scale (cm-1) set by reference to the scattering from pure H2O at 22°C. I(0) values must be corrected for the shear flow sample cell by a 2.05 multiplicative factor. Data were acquired in SEC-SAXS mode, summed from 12 x 1 second measurement frames where Rg was flat and at a maximum. The average concentration over the frames was 0.959 mg/mL, spanning values from 0.74 - 1.1 mg/mL. Sample concentrations were measured by A280 (extinction coefficient for 1 mg/mL, 10 mm pathlength; E0.1% = 0.178), corrected for the 3.1 mm path-length of the UV measurement cell. MULCh was used to calculate the partial specific volume (0.716 mL/g) and X-ray contrast (Δρ = 3.093e10 cm-2). The protein is a monomer in the solution conditions measured, experimental molecular weight provided includes the 4 bound calcium ions. The multi-state (Multi-FoXS, 1-state and 2-sate fits) and ensemble model (EOM, lower panel) fits to the data assumed flexibility in residues 77-81, the region identified by NMR relaxation as highly mobile in the helix connecting the N- and C-terminal CaM domains. The distribution of Rg values shown for the EOM fit represents the pool (gray) and the optimized ensemble (blue).
Storage temperature = UNKNOWN
Tags:
benchmark
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