A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions

Clement Blanchet.

SASDCQ8 – Bovine serum albumin (BSA)

Bovine serum albumin
MWexperimental 62 kDa
MWexpected 66 kDa
log I(s) 8.15×105 8.15×104 8.15×103 8.15×102
Bovine serum albumin small angle scattering data  s, nm-1
ln I(s)
Bovine serum albumin Guinier plot ln 8.16×105 Rg: 2.8 nm 0 (2.8 nm)-2 s2
(sRg)2I(s)/I(0)
Bovine serum albumin Kratky plot 1.104 0 √3 sRg

Data validation

Fits and models

log I(s)
 s, nm-1
Bovine serum albumin PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Bovine serum albumin DAMMIN model
Synchrotron SAXS data from solutions of bovine serum albumin (BSA) in 50 mM Tris, pH 7.4 were collected at the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany) using an Eiger 4M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 2.82 mg/ml was measured at 20°C. A single 1.3 millisecond frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data were scaled to the protein concentration.

Tags: benchmark
Bovine serum albumin (BSA)
Mol. type   Protein
Organism   Bos taurus
Olig. state   Monomer
Mon. MW   66.5 kDa
 
UniProt   P02769 (25-607)
Sequence   FASTA
 
PDB ID   3V03