Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains.

Lambert JP, Picaud S, Fujisawa T, Hou H, Savitsky P, Uusküla-Reimand L, Gupta GD, Abdouni H, Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC, Mol Cell (2018) Europe PMC

SASDCR2 – Bromodomain-containing protein 4 (BRD4) tandem bromodomains

Bromodomain-containing protein 4
MWexperimental 47 kDa
MWexpected 47 kDa
VPorod 251 nm3
log I(s) 1.03×10-1 1.03×10-2 1.03×10-3 1.03×10-4
Bromodomain-containing protein 4 small angle scattering data  s, nm-1
ln I(s)
Bromodomain-containing protein 4 Guinier plot ln 1.04×10-1 Rg: 6.3 nm 0 (6.3 nm)-2 s2
(sRg)2I(s)/I(0)
Bromodomain-containing protein 4 Kratky plot 1.104 0 √3 sRg
p(r)
Bromodomain-containing protein 4 pair distance distribution function Rg: 6.8 nm 0 Dmax: 22 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Bromodomain-containing protein 4 DAMMIN model
Synchrotron SAXS data from solutions of BRD4 tandem bromodomains in 20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 3.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). In-line continuous flow HPLC-SAXS was employed at 10°C with a sample injection concentration of 13 mg/ml onto a Shodex 404 column. 12 successive 10 second frames were collected through the HPLC-elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves underwent single-value decomposition to produce the average-median scattering data displayed in this entry.

Bromodomain-containing protein 4 (BRD4)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   47.2 kDa
 
UniProt   O60885 (44-460)
Sequence   FASTA