SASDCR6 – Rap guanine nucleotide exchange factor 3 (isoform3) - binary form with cAMP

Rap guanine nucleotide exchange factor 3 (dimer)

MW^I(0)	178	kDa
MW^expected	200	kDa
V^Porod	415	nm³

log I(s) 1.97×10⁰ 1.97×10^-1 1.97×10^-2 1.97×10^-3

Rap guanine nucleotide exchange factor 3 (dimer) small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Rap guanine nucleotide exchange factor 3 (dimer) Guinier plot

ln 1.97×10⁰ R_g: 5.3 nm 0 (5.3 nm)^-2 s²

(sR_g)²I(s)/I(0)

Rap guanine nucleotide exchange factor 3 (dimer) Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 5.2 nm 0 D_max: 15.7 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.7

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.763
0.930

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Rap guanine nucleotide exchange factor 3 (dimer) CORAL model

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Rap guanine nucleotide exchange factor 3 (dimer) DAMMIF model

SAXS data from solutions of Rap guanine nucleotide exchange factor 3 (isoform3) - binary form with cAMP - in 1mM EDTA, 10mM DTT, 500mM NaCl, 1mM cAMP, 10mM Tris, pH 9 were collected using a Rigaku BioSAXS-1000 instrument at the Sealy Center For Structural Biology, UTMB-G (Galveston, TX, USA) equipped with a Kratky-2D Rigaku BioSAXS-1000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). A sample concentration of 4 mg/ml was measured at 10°C. SAXS data were acquired from 4 successive 1800 second frames. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted to generate the data displayed for this entry.

Rap guanine nucleotide exchange factor 3 (dimer) (EPAC1B (dimer))
Mol. type		Protein
Organism		Homo sapiens
Olig. state		Dimer
Mon. MW		100.0 kDa

UniProt		O95398-3 (2-881)
Sequence		FASTA

PDB ID		4ZF7