Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors.

de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V, Mol Cell 69(6):979-992.e6 (2018) Europe PMC

SASDCU8 – Uncharacterized protein CTHT_0072540 (WD40) from Chaetomium thermophilum

hypothetical protein CTHT_0072540
MWI(0) 56 kDa
MWexpected 35 kDa
VPorod 68 nm3
log I(s) 1.47×103 1.47×102 1.47×101 1.47×100
hypothetical protein CTHT_0072540 small angle scattering data  s, nm-1
ln I(s)
hypothetical protein CTHT_0072540 Guinier plot ln 1.48×103 Rg: 2.3 nm 0 (2.3 nm)-2 s2
(sRg)2I(s)/I(0)
hypothetical protein CTHT_0072540 Kratky plot 1.104 0 √3 sRg
p(r)
hypothetical protein CTHT_0072540 pair distance distribution function Rg: 2.2 nm 0 Dmax: 5.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
hypothetical protein CTHT_0072540 SASREF model
log I(s)
 s, nm-1
hypothetical protein CTHT_0072540 DAMMIN model
Synchrotron SAXS data from solutions of Uncharacterized protein CTHT_0072540 (WD40) from Chaetomium thermophilum in 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH 7.5 were collected on the P12 beam line at the PETRAIII the storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1.5 and 5 mg/ml were measured at 25°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.

Storage temperature = UNKNOWN

hypothetical protein CTHT_0072540
Mol. type   Protein
Organism   Chaetomium thermophilum
Olig. state   Monomer
Mon. MW   34.6 kDa
 
UniProt   G0SFY0 (155-480)
Sequence   FASTA