Ca2+ - Induced Structural Change of Multi-Domain Collagen Binding Segments of Collagenases ColG and ColH from Hathewaya histolytica

Christopher E Ruth, University of Arkansas Dissertation - (2018) URL

SASDCV4 – Collagenase ColG s2s3as3b at pCa 6

Collagenase ColG segement s2s3as3b
MWexperimental 37 kDa
MWexpected 37 kDa
VPorod 41 nm3
log I(s) 2.41×100 2.41×10-1 2.41×10-2 2.41×10-3
Collagenase ColG segement s2s3as3b small angle scattering data  s, nm-1
ln I(s)
Collagenase ColG segement s2s3as3b Guinier plot ln 2.41×100 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
Collagenase ColG segement s2s3as3b Kratky plot 1.104 0 √3 sRg
p(r)
Collagenase ColG segement s2s3as3b pair distance distribution function Rg: 3.9 nm 0 Dmax: 15.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Collagenase ColG segement s2s3as3b DAMMIF model
Synchrotron SAXS data from solutions of Collagenase ColG s2s3as3b at pCa 6 in 10mM HEPES 100mM NaCl 0.2mM EGTA, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 3 and 5 mg/ml were measured at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve. The model represents the averaged spatial representation of the protein (DAMFILT occupancy and volume-corrected bead model).

X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

Collagenase ColG segement s2s3as3b (s2s3as3b)
Mol. type   Protein
Organism   Hathewaya histolytica
Olig. state   Monomer
Mon. MW   37.2 kDa
 
UniProt   Q9X721 (787-1118)
Sequence   FASTA