Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls.

Sun Y, Roznowski AP, Tokuda JM, Klose T, Mauney A, Pollack L, Fane BA, Rossmann MG, Proc Natl Acad Sci U S A 114(52):13708-13713 (2017) Europe PMC

SASDCV9 – Bacteriophage phi-X174 - ~45s after mixing with LPS

Bacteriophage phi-X174
MWexpected 0 kDa
log I(s) 3.67×101 3.67×100 3.67×10-1 3.67×10-2
Bacteriophage phi-X174 small angle scattering data  s, nm-1
ln I(s)
Bacteriophage phi-X174 Guinier plot ln 3.67×101 Rg: 12.7 nm 0 (12.7 nm)-2 s2
(sRg)2I(s)/I(0)
Bacteriophage phi-X174 Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Bacteriophage phi-X174 ~45 s after mixing with Lipopolysaccharide (LPS) in 0.15 mg/mL LPS, 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH 7.4 were collected on the BioSAXS G1 beam line at the Cornell High Energy Synchrotron Source (CHESS; Ithaca, NY, USA) using a Pilatus Pilatus 200K detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1109 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 0.10 mg/ml phage was measured at 33°C. 10 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Note: Due to the size of the capsids, the Rg and I(0) obtained from Guinier analysis should be interpreted as approximated estimates only.

Bacteriophage phi-X174
Mol. type   Protein
Olig. state   Monomer
Mon. MW   0.2 kDa
Sequence   FASTA