Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.

Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C, Structure 26(2):345-355.e5 (2018) Europe PMC

SASDCX6 – T2 SapNP (SapA:POPS) (SEC-SAXS)

saposin-a
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine
Mechanosensitive channel T2
MWexperimental 323 kDa
MWexpected 286 kDa
VPorod 686 nm3
log I(s) 2.56×103 2.56×102 2.56×101 2.56×100
saposin-a 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine Mechanosensitive channel T2 small angle scattering data  s, nm-1
ln I(s)
saposin-a 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine Mechanosensitive channel T2 Guinier plot ln 2.56×103 Rg: 5.4 nm 0 (5.4 nm)-2 s2
(sRg)2I(s)/I(0)
saposin-a 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine Mechanosensitive channel T2 Kratky plot 1.104 0 √3 sRg
p(r)
saposin-a 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine Mechanosensitive channel T2 pair distance distribution function Rg: 5.4 nm 0 Dmax: 18 nm

Data validation

Fits and models

log I(s)
 s, nm-1
saposin-a 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine Mechanosensitive channel T2 DAMMIF model
Synchrotron SAXS data from solutions of T2 SapNP (SapA:POPS) (SEC-SAXS) in PBS, pH 7.4 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 10°C. 3600 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Concentration = UNKNOWN

saposin-a (sapA)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   9.1 kDa
 
UniProt   P07602 (60-140)
Sequence   FASTA
 
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS)
Mol. type   Other
Organism   synthetic construct
Olig. state   Other
Mon. MW   0.8 kDa
Mechanosensitive channel T2 (T2)
Mol. type   Protein
Organism   Thermoplasma volcanium
Olig. state   Heptamer
Mon. MW   32.9 kDa
 
UniProt   Q97AH5 (1-197)
Sequence   FASTA