Rev7 dimerization is important for assembly and function of the Rev1/PolĪ¶ translesion synthesis complex.

Rizzo AA, Vassel FM, Chatterjee N, D'Souza S, Li Y, Hao B, Hemann MT, Walker GC, Korzhnev DM, Proc Natl Acad Sci U S A 115(35):E8191-E8200 (2018) Europe PMC

SASDCY8 – Human Rev7 monomer (R124A mutant) in complex with Rev3 peptide @ 8mg/mL

Mitotic spindle assembly checkpoint protein MAD2B
DNA polymerase zeta catalytic subunit
MWI(0) 27 kDa
MWexpected 28 kDa
VPorod 46 nm3
log I(s) 1.38×10-1 1.38×10-2 1.38×10-3 1.38×10-4
Mitotic spindle assembly checkpoint protein MAD2B DNA polymerase zeta catalytic subunit small angle scattering data  s, nm-1
ln I(s)
Mitotic spindle assembly checkpoint protein MAD2B DNA polymerase zeta catalytic subunit Guinier plot ln 1.38×10-1 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
Mitotic spindle assembly checkpoint protein MAD2B DNA polymerase zeta catalytic subunit Kratky plot 1.104 0 √3 sRg
p(r)
Mitotic spindle assembly checkpoint protein MAD2B DNA polymerase zeta catalytic subunit pair distance distribution function Rg: 2.1 nm 0 Dmax: 7.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Mitotic spindle assembly checkpoint protein MAD2B DNA polymerase zeta catalytic subunit PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of Human Rev7 monomer (R124A mutant) in complex with Rev3 peptide @ 8mg/mL in 20 mM Tris, 150 mM NaCl, 1 mM EDTA, 10 mM DTT, 5% glycerol, pH 8.4 were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS) storage ring (Ithaca, NY, USA) using a Finger Lakes CCD detector at a wavelength of λ = 0.1267 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 8.00 mg/ml was measured at 4°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN

Mitotic spindle assembly checkpoint protein MAD2B (hRev7-R124A)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   24.3 kDa
 
UniProt   Q9UI95 (1-211)
Sequence   FASTA
 
DNA polymerase zeta catalytic subunit (Rev3-RBM2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   3.3 kDa
 
UniProt   O60673 (1988-2014)
Sequence   FASTA
 
PDB ID   6BC8