Structure and dynamics of the RNAPII CTDsome with Rtt103.

Jasnovidova O, Klumpler T, Kubicek K, Kalynych S, Plevka P, Stefl R, Proc Natl Acad Sci U S A 114(42):11133-11138 (2017) Europe PMC

SASDCZ2 – Yeast CTDsome: transcription termination factor Rtt103p/C-terminal domain of DNA-directed RNA polymerase II subunit RPB1 (Rtt103 1-246-phospho-mimetic complex)

Regulator of Ty1 transposition protein 103
DNA-directed RNA polymerase II subunit RPB1
MWexperimental 185 kDa
MWexpected 70 kDa
VPorod 561 nm3
log I(s) 1.80×10-1 1.80×10-2 1.80×10-3 1.80×10-4
Regulator of Ty1 transposition protein 103 DNA-directed RNA polymerase II subunit RPB1 small angle scattering data  s, nm-1
ln I(s)
Regulator of Ty1 transposition protein 103 DNA-directed RNA polymerase II subunit RPB1 Guinier plot ln 1.80×10-1 Rg: 5.3 nm 0 (5.3 nm)-2 s2
(sRg)2I(s)/I(0)
Regulator of Ty1 transposition protein 103 DNA-directed RNA polymerase II subunit RPB1 Kratky plot 1.104 0 √3 sRg
p(r)
Regulator of Ty1 transposition protein 103 DNA-directed RNA polymerase II subunit RPB1 pair distance distribution function Rg: 5.8 nm 0 Dmax: 21 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Regulator of Ty1 transposition protein 103 DNA-directed RNA polymerase II subunit RPB1 CORAL model
log I(s)
 s, nm-1
Regulator of Ty1 transposition protein 103 DNA-directed RNA polymerase II subunit RPB1 CORAL model
log I(s)
 s, nm-1
Regulator of Ty1 transposition protein 103 DNA-directed RNA polymerase II subunit RPB1 CORAL model
SAXS data from solutions of Rtt103 1-246-phospho-mimetic complex in 25 mM KH2PO4, 300 mM KCl, 10 mM β-mercaptoethanol, pH 6.5 were collected using a BioSAXS-1000 instrument at CEITEC (Brno, Czech Republic) equipped with a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Five successive 3600 second frames were collected at a sample temperature of 4°C. The data were normalized to the intensity of the transmitted beam and radially averaged and the corresponding scattering from the solvent-blank was subtracted to produce the scattering profile displayed in this entry.

Regulator of Ty1 transposition protein 103
Mol. type   Protein
Organism   Saccharomyces cerevisiae
Olig. state   Dimer
Mon. MW   28.6 kDa
 
UniProt   Q05543 (1-246)
Sequence   FASTA
 
DNA-directed RNA polymerase II subunit RPB1
Mol. type   Protein
Organism   Saccharomyces cerevisiae
Olig. state   Monomer
Mon. MW   12.8 kDa
 
UniProt   P04050
Sequence   FASTA
 
PDB ID   5M48
 
PDB ID   5M48