Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase.

Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V, J Mol Biol 430(8):1218-1234 (2018) Europe PMC

SASDCZ9 – Glyceraldehyde-3-phosphate dehydrogenase from C. reinhardtii

Glyceraldehyde-3-phosphate dehydrogenase
MWexperimental 148 kDa
MWexpected 148 kDa
VPorod 206 nm3
log I(s) 2.57×10-4 2.57×10-5 2.57×10-6 2.57×10-7
Glyceraldehyde-3-phosphate dehydrogenase small angle scattering data  s, nm-1
ln I(s)
Glyceraldehyde-3-phosphate dehydrogenase Guinier plot ln 2.57×10-4 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Glyceraldehyde-3-phosphate dehydrogenase Kratky plot 1.104 0 √3 sRg
p(r)
Glyceraldehyde-3-phosphate dehydrogenase pair distance distribution function Rg: 3.2 nm 0 Dmax: 9.0 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Glyceraldehyde-3-phosphate dehydrogenase MODELLER model
log I(s)
 s, nm-1
Glyceraldehyde-3-phosphate dehydrogenase MODELLER model
Synchrotron SAXS data from solutions of glyceraldehyde-3-phosphate dehydrogenase in 30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteine, pH 7.9, were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a CCD AVIEX detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.1033 nm (I(s) vs s where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 23.00 mg/ml was measured at 20°C. 250 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.

SEC column = UNKNOWN. Sample injection volume = UNKNOWN. Flow rate = UNKNOWN

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Mol. type   Protein
Organism   Chlamydomonas reinhardtii
Olig. state   Tetramer
Mon. MW   36.9 kDa
 
UniProt   A8HP84
Sequence   FASTA