Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP.

Slonimskiy YB, Maksimov EG, Lukashev EP, Moldenhauer M, Jeffries CM, Svergun DI, Friedrich T, Sluchanko NN, Biochim Biophys Acta 1859(5):382-393 (2018) Europe PMC

SASDD52 – Arthrospira fluorescence recovery protein AmaxFRP.1-106

Uncharacterized fluorescence recovery protein
MWI(0) 22 kDa
MWexpected 24 kDa
VPorod 35 nm3
log I(s) 1.61×10-2 1.61×10-3 1.61×10-4 1.61×10-5
Uncharacterized fluorescence recovery protein small angle scattering data  s, nm-1
ln I(s)
Uncharacterized fluorescence recovery protein Guinier plot ln 1.61×10-2 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
Uncharacterized fluorescence recovery protein Kratky plot 1.104 0 √3 sRg
p(r)
Uncharacterized fluorescence recovery protein pair distance distribution function Rg: 2.8 nm 0 Dmax: 9.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Uncharacterized fluorescence recovery protein GASBOR model
log I(s)
 s, nm-1
Uncharacterized fluorescence recovery protein ITASSER model
Synchrotron SAXS data from solutions of Arthrospira fluorescence recovery protein AmaxFRP.1-106 in 20 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 2 mM dithiothreitol, 3 % v/v glycerol, pH 7.6 were collected at the EMBL-P12 bioSAXS beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 5.77 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data scaled for protein concentration.

Additional GASBOR models, with spatial alignments and the NSD estimate can be located in the full entry zip archive.

Uncharacterized fluorescence recovery protein (FRP)
Mol. type   Protein
Organism   Arthrospira maxima CS-328
Olig. state   Dimer
Mon. MW   12.2 kDa
 
UniProt   B5W3T4 (1-106)
Sequence   FASTA