| MWI(0) | 41 | kDa |
| MWexpected | 52 | kDa |
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log I(s)
2.48×10-2
2.48×10-3
2.48×10-4
2.48×10-5
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s, nm-1
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Self-Assembling Supramolecular Nanostructures Constructed from de Novo Extender Protein Nanobuilding Blocks.
Kobayashi N, Inano K, Sasahara K, Sato T, Miyazawa K, Fukuma T, Hecht MH, Song C, Murata K, Arai R, ACS Synth Biol 7(5):1381-1394 (2018) Europe PMCSASDD56 – Artificially designed de novo protein esPN-Block (FL4) heterocomplex, e1s2 (FL4)
stopper PN-Block (WA20)extender PN-Block (FL4)
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Fits and models
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Synchrotron SAXS data from solutions of an artificially designed de novo protein, esPN-Block (FL4) heterocomplex e1s2 (FL4), in 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% v/v glycerol, pH 7.5 were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Acceleration Research Organization (KEK; Tsukuba, Japan) using a Pilatus 2M detector at a sample-detector distance of 1.1 m and at a wavelength of λ = 0.1488 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.50 mg/ml was measured at 20°C. 30 successive 5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Storage temperature = UNKNOWN |
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