Grinter R,
Hay ID,
Song J,
Wang J,
Teng D,
Dhanesakaran V,
Wilksch JJ,
Davies MR,
Littler D,
Beckham SA,
Henderson IR,
Strugnell RA,
Dougan G,
Lithgow T,
PLoS Biol
16(8):e2006026
(2018)
Europe PMC
Synchrotron SAXS data from solutions of ferredoxin protease, FusC, E83A mutant + 150 µM Arabidopsis ferredoxin in 20 mM Tris, 150 mM NaCl, pH 7.8 were collected on the SAXS/WAXS beam line at the Australian Synchrotron storage ring (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.3 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The FusC E83A mutant (30 µM) in the presence of 150 µM Arabidopsis ferredoxin. The background subtraction included 150 µM Arabidopsis ferredoxin in buffer.