NAD+ Promotes Assembly of the Active Tetramer of Aldehyde Dehydrogenase 7A1.

Korasick DA, White TA, Chakravarthy S, Tanner JJ, FEBS Lett (2018) Europe PMC

SASDD79 – High load concentration of alpha-aminoadipic semialdehyde dehydrogenase ALDH7A1 with nicotinamide adenine dinucleotide (NAD) collected by SEC-SAXS

Alpha-aminoadipic semialdehyde dehydrogenase
MWexperimental 179 kDa
MWexpected 222 kDa
VPorod 277 nm3
log I(s) 1.23×102 1.23×101 1.23×100 1.23×10-1
Alpha-aminoadipic semialdehyde dehydrogenase small angle scattering data  s, nm-1
ln I(s)
Alpha-aminoadipic semialdehyde dehydrogenase Guinier plot ln 1.23×102 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Alpha-aminoadipic semialdehyde dehydrogenase Kratky plot 1.104 0 √3 sRg

Data validation

Fits and models

log I(s)
 s, nm-1
Alpha-aminoadipic semialdehyde dehydrogenase PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of alpha-aminoadipic semialdehyde dehydrogenase (ALDH7A1) in 50 mM Tris, 50 mM NaCl, 0.5 mM DTT, 5% (v/v) glycerol, 1 mM NAD, pH 7.8 were collected using SEC-SAXS on the BioCAT 18ID beam line at the Advanced Photon Source (APS; Argonne, IL, USA) using a Pilatus 100K detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Data were recorded from samples at 22°C.

Column type: Wyatt WTC 030 S5; flow rate: 0.8 mL/min; injection volume: 250 - 500 µL

Alpha-aminoadipic semialdehyde dehydrogenase (ALDH7A1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Tetramer
Mon. MW   55.6 kDa
 
UniProt   P49419
Sequence   FASTA
 
PDB ID   4ZUK