Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering.

Cai K, Frederick RO, Dashti H, Markley JL, Structure 26(8):1127-1136.e4 (2018) Europe PMC

SASDDB3 – Human mitochondrial cysteine desulfurase (NFS1, ISD11 and Acp heterodimer complex)

Cysteine desulfurase, mitochondrial
LYR motif-containing protein 4
Acyl carrier protein
MWexperimental 125 kDa
MWexpected 135 kDa
VPorod 204 nm3
log I(s) 9.31×102 9.31×101 9.31×100 9.31×10-1
Cysteine desulfurase, mitochondrial LYR motif-containing protein 4 Acyl carrier protein small angle scattering data  s, nm-1
ln I(s)
Cysteine desulfurase, mitochondrial LYR motif-containing protein 4 Acyl carrier protein Guinier plot ln 9.32×102 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Cysteine desulfurase, mitochondrial LYR motif-containing protein 4 Acyl carrier protein Kratky plot 1.104 0 √3 sRg
p(r)
Cysteine desulfurase, mitochondrial LYR motif-containing protein 4 Acyl carrier protein pair distance distribution function Rg: 3.7 nm 0 Dmax: 11.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Cysteine desulfurase, mitochondrial LYR motif-containing protein 4 Acyl carrier protein PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Cysteine desulfurase, mitochondrial LYR motif-containing protein 4 Acyl carrier protein PDB (PROTEIN DATA BANK) model
SAXS data from solutions of human mitochondrial cysteine desulfurase (NFS1, ISD11 and Acp heterodimer complex) in 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH 7.5 were collected on a Bruker Nanostar instrument (Madison, WI, USA) using a MikroGap Vantec-2000 detector at a sample-detector distance of 1 m and at a wavelength of λ = 0.15418 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2 and 10 mg/ml were measured at 25°C. Four successive 3600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Solution SAXS study the human mitochondrial cysteine desulfurase, which consists of three components: NFS1, ISD11 and Acp and forms a homodimer.

Cysteine desulfurase, mitochondrial (NFS1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   44.9 kDa
 
UniProt   Q9Y697 (53-457)
Sequence   FASTA
 
LYR motif-containing protein 4 (ISD11)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   11.6 kDa
 
UniProt   Q9HD34
Sequence   FASTA
 
Acyl carrier protein
Mol. type   Protein
Organism   Escherichia coli
Olig. state   Dimer
Mon. MW   11.5 kDa
 
UniProt   P0A6A8
Sequence   FASTA
 
PDB ID   5WGB
 
PDB ID   5USR