The 3-D structure of VNG0258H/RosR - A haloarchaeal DNA-binding protein in its ionic shell.

Kutnowski N, Shmuely H, Dahan I, Shmulevich F, Davidov G, Shahar A, Eichler J, Zarivach R, Shaanan B, J Struct Biol (2018) Europe PMC

SASDDC9 – Structure of Halobacterium salinarum VNG0258H/RosR in the presence of 2 M RbCl

VNG0258H/RosR
MWexperimental 42 kDa
MWexpected 29 kDa
VPorod 89 nm3
log I(s) 1.30×101 1.30×100 1.30×10-1 1.30×10-2
VNG0258H/RosR small angle scattering data  s, nm-1
ln I(s)
VNG0258H/RosR Guinier plot ln 1.30×101 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
VNG0258H/RosR Kratky plot 1.104 0 √3 sRg
p(r)
VNG0258H/RosR pair distance distribution function Rg: 3.1 nm 0 Dmax: 9.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
VNG0258H/RosR DAMMIN model
VNG0258H/RosR DAMFILT model
Synchrotron SAXS data from solutions of Halobacterium salinarum VNG0258H/RosR in 50 mM HEPES, 2 M RbCl, 0.02% NaN3, pH 7 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.10 mg/ml was measured at 25°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Individual (top) and spatially averaged (DAMFILT, bottom) ab initio models are displayed.

VNG0258H/RosR (VNG0258H/RosR)
Mol. type   Protein
Organism   Halobacterium salinarum NRC-1
Olig. state   Dimer
Mon. MW   14.5 kDa
 
UniProt   Q9HSF4 (1-116)
Sequence   FASTA