The molecular basis for Apolipoprotein E4 as the major risk factor for late onset Alzheimer's disease.

Raulin AC, Kraft L, Al-Hilaly YK, Xue WF, McGeehan JE, Atack JR, Serpell L, J Mol Biol (2019) Europe PMC

SASDDD7 – Apolipoprotein E2

Apolipoprotein E2
MWexperimental 144 kDa
MWexpected 139 kDa
VPorod 400 nm3
log I(s) 2.76×10-1 2.76×10-2 2.76×10-3 2.76×10-4
Apolipoprotein E2 small angle scattering data  s, nm-1
ln I(s)
Apolipoprotein E2 Guinier plot ln 2.76×10-1 Rg: 5.6 nm 0 (5.6 nm)-2 s2
(sRg)2I(s)/I(0)
Apolipoprotein E2 Kratky plot 1.104 0 √3 sRg
p(r)
Apolipoprotein E2 pair distance distribution function Rg: 5.8 nm 0 Dmax: 19.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of human apolipoprotein E2 in 20 mM HEPES, 300 mM NaCl, pH 8 were collected on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 3.9 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Seven successive 3 second frames were collected at a sample temperature of 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample concentration: UNKNOWN. The experimental molecular weight (MW) quoted in this entry (144 kDa) was determined using multi-angle laser light scattering (MALLS). Analytical ultracentrifugation provides a MW estimate of 122 kDa.

Apolipoprotein E2 (ApoE2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Tetramer
Mon. MW   34.6 kDa
 
UniProt   P02649 (19-317)
Sequence   FASTA