| MWexperimental | 25 | kDa |
| MWexpected | 26 | kDa |
| VPorod | 37 | nm3 |
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log I(s)
1.60×10-2
1.60×10-3
1.60×10-4
1.60×10-5
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s, nm-1
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Integrin α6β4 Recognition of a Linear Motif of Bullous Pemphigoid Antigen BP230 Controls Its Recruitment to Hemidesmosomes.
Manso JA, Gómez-Hernández M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda JM, Structure 27(6):952-964.e6 (2019) Europe PMCSASDDE8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4)
Integrin beta-4 fragment with final part of the connecting segment and the third and fourth FnIII domains|
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Fits and models
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Rg, nm
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Synchrotron SAXS data from solutions of the integrin beta4 fragment in 20 mM sodium phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.9 and 14.9 mg/ml were measured at 10°C. 30 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Synchrotron SAXS data from solutions of a fragment of the cytoplasmic region of the human integrin Beta4 subunit. This fragment includes the final part of a region named the "connecting segment" the third and fourth fibronectin type III domains (FnIII-3,4) Samples at different solute concentration in the range 0.9 – 14.9 mg/ml were obtained by 2-fold serial dilution and were measured. |
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