Crystal structure of an Lrs14-like archaeal biofilm regulator from Sulfolobus acidocaldarius.

Vogt MS, Völpel SL, Albers SV, Essen LO, Banerjee A, Acta Crystallogr D Struct Biol 74(Pt 11):1105-1114 (2018) Europe PMC

SASDDH3 – Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2)

Transcriptional regulator Lrs14-like protein
MWI(0) 39 kDa
MWexpected 33 kDa
VPorod 57 nm3
log I(s) 2.41×101 2.41×100 2.41×10-1 2.41×10-2
Transcriptional regulator Lrs14-like protein small angle scattering data  s, nm-1
ln I(s)
Transcriptional regulator Lrs14-like protein Guinier plot ln 2.42×101 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Transcriptional regulator Lrs14-like protein Kratky plot 1.104 0 √3 sRg
p(r)
Transcriptional regulator Lrs14-like protein pair distance distribution function Rg: 2.8 nm 0 Dmax: 10 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Transcriptional regulator Lrs14-like protein DAMMIF model
log I(s)
 s, nm-1
Transcriptional regulator Lrs14-like protein PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Synchrotron SAXS data from solutions of Archaeal biofilm regulator 2 (AbfR2) in 300 mM NaCl, 20 mM HEPES, pH 7.5, were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.83 mg/ml was measured at 4°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The associated atomic model structure is included for comparative purposes only, i.e., to compare the overall topology of the atomic model with the solution scattering and the DAMMIF bead model. Two fits displayed for the atomic model were calculated using CRYSOL (top) and SASREF (bottom).

Transcriptional regulator Lrs14-like protein (AbfR2)
Mol. type   Protein
Organism   Sulfolobus acidocaldarius
Olig. state   Dimer
Mon. MW   16.4 kDa
 
UniProt   Q4J9G1 (1-123)
Sequence   FASTA
 
PDB ID   6CMV