Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein.

Yerabham ASK, Müller-Schiffmann A, Ziehm T, Stadler A, Köber S, Indurkhya X, Marreiros R, Trossbach SV, Bradshaw NJ, Prikulis I, Willbold D, Weiergräber OH, Korth C, PLoS One 13(1):e0191162 (2018) Europe PMC

SASDDM7 – Complex of disrupted-in-Schizophrenia 1 protein (DISC1 12D2) and anti-DISC1 single-domain camelid antibody VHH B5

anti-DISC1 single-domain camelid antibody VHH B5
Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836
MWI(0) 36 kDa
MWexpected 33 kDa
VPorod 77 nm3
log I(s) 6.80×101 6.80×100 6.80×10-1 6.80×10-2
anti-DISC1 single-domain camelid antibody VHH B5 Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 small angle scattering data  s, nm-1
ln I(s)
anti-DISC1 single-domain camelid antibody VHH B5 Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 Guinier plot ln 6.81×101 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
anti-DISC1 single-domain camelid antibody VHH B5 Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 Kratky plot 1.104 0 3 sRg
p(r)
anti-DISC1 single-domain camelid antibody VHH B5 Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 pair distance distribution function Rg: 3.1 nm 0 Dmax: 10.4 nm

Data validation


Fits and models


log I(s)
 s, nm-1
anti-DISC1 single-domain camelid antibody VHH B5 Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 DAMMIF model
anti-DISC1 single-domain camelid antibody VHH B5 Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 DAMMIF model

Synchrotron SAXS data from solutions of the complex formed between the disrupted-in-Schizophrenia 1 protein (DISC1 12D2) and the anti-DISC1 single-domain camelid antibody VHH B5 in 25 mM Tris-HCl, 150 mM NaCl, 1mM DTT, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.35 mg/ml was measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN

anti-DISC1 single-domain camelid antibody VHH B5 (VHH B5)
Mol. type   Protein
Olig. state   Monomer
Mon. MW   13.9 kDa
Sequence   FASTA
 
Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 (DISC1 12D2)
Mol. type   Protein
Olig. state   Monomer
Mon. MW   19.4 kDa
 
UniProt   Q9NRI5 (691-836)
Sequence   FASTA