Structure of the lipoprotein lipase-GPIHBP1 complex that mediates plasma triglyceride hydrolysis.

Birrane G, Beigneux AP, Dwyer B, Strack-Logue B, Kristensen KK, Francone OL, Fong LG, Mertens HDT, Pan CQ, Ploug M, Young SG, Meiyappan M, Proc Natl Acad Sci U S A (2018) Europe PMC

SASDDM9 – lipoprotein lipase–GPIHBP1 complex

Lipoprotein lipase
Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1
MWI(0) 152 kDa
MWexpected 130 kDa
VPorod 319 nm3
log I(s) 7.40×10-2 7.40×10-3 7.40×10-4 7.40×10-5
Lipoprotein lipase Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 small angle scattering data  s, nm-1
ln I(s)
Lipoprotein lipase Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 Guinier plot ln 7.40×10-2 Rg: 5.5 nm 0 (5.5 nm)-2 s2
(sRg)2I(s)/I(0)
Lipoprotein lipase Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 Kratky plot 1.104 0 √3 sRg
p(r)
Lipoprotein lipase Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 pair distance distribution function Rg: 5.6 nm 0 Dmax: 19 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Lipoprotein lipase Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 DAMMIF model
Lipoprotein lipase Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 DAMFILT model
Synchrotron SAXS data from solutions of lipoprotein lipase–GPIHBP1 complex in 10 mM BisTris, 150 mM NaCl, 4 mM CaCl2, 10% glycerol, pH 7.4 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.72 mg/ml was measured at 25°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Lipoprotein lipase (hLPL)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   50.3 kDa
 
UniProt   P06858 (29-475)
Sequence   FASTA
 
Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 (GPIHBP1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   14.7 kDa
Sequence   FASTA