| MWexperimental | 182 | kDa |
| MWexpected | 132 | kDa |
| VPorod | 248 | nm3 |
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log I(s)
1.56×103
1.56×102
1.56×101
1.56×100
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s, nm-1
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Structures of Proline Utilization A (PutA) Reveal the Fold and Functions of the Aldehyde Dehydrogenase Superfamily Domain of Unknown Function.
Luo M, Gamage TT, Arentson BW, Schlasner KN, Becker DF, Tanner JJ, J Biol Chem 291(46):24065-24075 (2016) Europe PMCSASDDN2 – Sinorhizobium meliloti Proline Utilization A (PutA) at 3.00 mg/ml
Sinorhizobium meliloti (SmPutA)|
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Fits and models
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Synchrotron SAXS data from solutions of Sinorhizobium meliloti proline utilization A (PutA) in 50 mM Tris, 1% (v/v) glycerol, 0.5 mM THP and 50 mM NaCl, pH 7.8, were collected on the 12.3.1 SIBYLS beam line at the Advanced Light Source storage ring (ALS; Berkeley, CA, USA) using a MAR 165 CCD detector at a wavelength of λ = 0.1127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The fit to the SAXS data is shown for a volume-fraction weighted mixture of monomers and dimers. The volume fraction of monomer and dimer are 0.67 and 0.33, respectively. |
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