M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision repair.

Khanam T, Afsar M, Shukla A, Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R, Nucleic Acids Res (2020) Europe PMC

SASDDN9 – Mycobacterium tuberculosis DNA Ligase A

DNA ligase A
MWexperimental 121 kDa
MWexpected 76 kDa
VPorod 870 nm3
log I(s) 1.08×101 1.08×100 1.08×10-1 1.08×10-2
DNA ligase A small angle scattering data  s, nm-1
ln I(s)
DNA ligase A Guinier plot ln 1.08×101 Rg: 5.2 nm 0 (5.2 nm)-2 s2
(sRg)2I(s)/I(0)
DNA ligase A Kratky plot 1.104 0 √3 sRg
p(r)
DNA ligase A pair distance distribution function Rg: 5.2 nm 0 Dmax: 16.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
DNA ligase A DAMFILT model
Synchrotron SAXS data from solutions of Mycobacterium tuberculosis DNA Ligase A in 50 mM Tris-HCl 200 mM NaCl 2mM β-mercaptoethanol, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 1.1 m and at a wavelength of λ = 0.081 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.30 mg/ml was measured at 10°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The model displayed in this entry is a DAMFILT averaged spatial representation obtained from an unknown number of individual DAM reconstructions.

DNA ligase A (LigA)
Mol. type   Protein
Organism   Mycobacterium tuberculosis
Olig. state   Monomer
Mon. MW   76.1 kDa
 
UniProt   P9WNV1 (1-691)
Sequence   FASTA