Redox Modulation of Oligomeric State in Proline Utilization A.

Korasick DA, Campbell AC, Christgen SL, Chakravarthy S, White TA, Becker DF, Tanner JJ, Biophys J 114(12):2833-2843 (2018) Europe PMC

SASDDP3 – N-propargyl glycine-Inactivated Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) collected by SEC-SAXS

Bifunctional protein PutA
MWexperimental 250 kDa
MWexpected 215 kDa
VPorod 324 nm3
log I(s) 3.77×102 3.77×101 3.77×100 3.77×10-1
Bifunctional protein PutA small angle scattering data  s, nm-1
ln I(s)
Bifunctional protein PutA Guinier plot ln 3.78×102 Rg: 4.6 nm 0 (4.6 nm)-2 s2
(sRg)2I(s)/I(0)
Bifunctional protein PutA Kratky plot 1.104 0 √3 sRg
p(r)
Bifunctional protein PutA pair distance distribution function Rg: 4.7 nm 0 Dmax: 14.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Bifunctional protein PutA PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Bifunctional protein PutA PDB (PROTEIN DATA BANK) model
Bifunctional protein PutA PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of N-propargyl glycine-Inactivated Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) were collected using SEC-SAXS in 50 mM Tris, 50 mM NaCl, 0.5 mM TCEP, 5% (v/v) glycerol, pH 7.8 on the BioCAT 18ID beam line at the Advanced Photon Source (APS, Argonne, IL, USA) using a Pilatus 100K detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The SEC-SAXS elution was collected at 22°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN. SEC column = UNKNOWN. Sample injection volume = UNKNOWN. Flow rate = UNKNOWN

Bifunctional protein PutA (BjPutA)
Mol. type   Protein
Organism   Bradyrhizobium diazoefficiens
Olig. state   Dimer
Mon. MW   107.6 kDa
 
UniProt   Q89E26
Sequence   FASTA
 
PDB ID   3HAZ