| MWI(0) | 184 | kDa |
| MWexpected | 180 | kDa |
| VPorod | 268 | nm3 |
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log I(s)
6.15×101
6.15×100
6.15×10-1
6.15×10-2
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s, nm-1
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Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3.
Scietti L, Chiapparino A, De Giorgi F, Fumagalli M, Khoriauli L, Nergadze S, Basu S, Olieric V, Cucca L, Banushi B, Profumo A, Giulotto E, Gissen P, Forneris F, Nat Commun 9(1):3163 (2018) Europe PMCSASDDW4 – Procollagen lysyl hydroxylase LH3 measured using SEC-SAXS
Procollagen lysyl hydroxylase LH3|
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Fits and models
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Synchrotron SAXS data from solutions of procollagen lysyl hydroxylase LH3 in 25 mM HEPES, 200 mM NaCl, pH 8 were measured at 20°C using SEC-SAXS collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) equipped with a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.50 mg/ml was injected onto the column. 75 successive 1 second frames were collected through the sample elution peak and processed using CHROMIXS that included the identification and subtraction of an appropriate solvent-blank.
The sample was injected at a load concentration of 3.5 mg/mL. The SEC column used was a Superdex 200 PC 3.2/300 Increase (GE Healthcare) at a flow rate 0.075 mL/min. |
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