| MWexperimental | 83 | kDa |
| MWexpected | 95 | kDa |
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log I(s)
2.99×10-3
2.99×10-4
2.99×10-5
2.99×10-6
|
s, nm-1
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Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems
Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K, (2018) DOISASDDX2 – EspG3-PE5/PPE4 complex from M. tuberculosis
EspG3 chaperone from Mycobacterium tuberculosisPE5 from Mycobacterium tuberculosis
PPE4 from Mycobacterium tuberculosis
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Fits and models
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Synchrotron SAXS
data from solutions of
EspG3-PE5/PPE4 complex from M. tuberculosis
in
20 mM HEPES pH 7.5, 150 mM NaCl, pH 7.5
were collected
on the
B21 beam line
at the Diamond Light Source storage ring
(Didcot, UK)
using a Pilatus 2M detector
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 1.22 mg/ml was measured.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Wavelength = UNKNOWN. Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN |
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