A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions.

Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J, ACS Comb Sci 20(4):197-202 (2018) Europe PMC

SASDDX4 – Wild-type Protein sex-lethal

Protein sex-lethal
MWI(0) 16 kDa
MWexpected 20 kDa
VPorod 26 nm3
log I(s) 1.19×103 1.19×102 1.19×101 1.19×100
Protein sex-lethal small angle scattering data  s, nm-1
ln I(s)
Protein sex-lethal Guinier plot ln 1.19×103 Rg: 2.0 nm 0 (2.0 nm)-2 s2
(sRg)2I(s)/I(0)
Protein sex-lethal Kratky plot 1.104 0 3 sRg
p(r)
Protein sex-lethal pair distance distribution function Rg: 2.0 nm 0 Dmax: 6.9 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of wild-type Sex-lethal protein in 10 mM KH2PO4, 50 mM NaCl, 10 mM DTT, pH 6 were collected on the P12 beam line at the PETRA III storage ring (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 25°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Protein sex-lethal (Sxl)
Mol. type   Protein
Organism   Drosophila melanogaster
Olig. state   Monomer
Mon. MW   19.8 kDa
 
UniProt   P19339 (123-294)
Sequence   FASTA