The structure of SALM5 suggests a dimeric assembly for the presynaptic RPTP ligand recognition.

Karki S, Paudel P, Sele C, Shkumatov AV, Kajander T, Protein Eng Des Sel (2018) Europe PMC

SASDDY2 – Leucine-rich repeat and fibronectin type-III domain-containing protein 4: Fragment SALM3 LRR-Ig

Leucine-rich repeat and fibronectin type-III domain-containing protein 4
MWexperimental 113 kDa
MWexpected 79 kDa
VPorod 183 nm3
log I(s) 1.75×101 1.75×100 1.75×10-1 1.75×10-2
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 small angle scattering data  s, nm-1
ln I(s)
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 Guinier plot ln 1.75×101 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 Kratky plot 1.104 0 √3 sRg
p(r)
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 pair distance distribution function Rg: 3.8 nm 0 Dmax: 12.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Leucine-rich repeat and fibronectin type-III domain-containing protein 4: Fragment SALM3 LRR-Ig Rg histogram Rg, nm
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 EOM/RANCH model
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 EOM/RANCH model
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 EOM/RANCH model
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 EOM/RANCH model
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 EOM/RANCH model
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 EOM/RANCH model
log I(s)
 s, nm-1
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 DAMMIN model
Synchrotron SAXS data from solutions of Leucine-rich repeat and fibronectin type-III domain-containing protein 4 (fragment SALM3 LRR-Ig) in 20 mM Tris HCl, 100 mM NaCl, 0.02% NaN3, pH 7.5, were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). A solute concentration of 12.00 mg/ml was injected onto a size exclusion chromatography column and the eluted sample and buffer were measured at 4.7°C. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted to produce the SAXS data displayed in this entry.

SEC column = UNKNOWN. Sample injection volume = UNKNOWN. Flow rate = UNKNOWN

Leucine-rich repeat and fibronectin type-III domain-containing protein 4 (LRR-Ig)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Dimer
Mon. MW   39.6 kDa
 
UniProt   Q80XU8 (17-371)
Sequence   FASTA