A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions.

Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J, ACS Comb Sci 20(4):197-202 (2018) Europe PMC

SASDDY4 – Protein sex-lethal mutant with 10GS-linker

Protein sex-lethal mutant

MW^experimental	16	kDa
MW^expected	20	kDa
V^Porod	27	nm³

log I(s) 2.26×10¹ 2.26×10⁰ 2.26×10^-1 2.26×10^-2

Protein sex-lethal mutant small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 2.27×10¹ R_g: 2.1 nm 0 (2.1 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

p(r)	R_g: 2.2 nm 0 D_max: 7.4 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.2

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.690
0.810

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Protein sex-lethal mutant with 10GS-linker Rg histogram

R_g, nm

Protein sex-lethal mutant EOM/RANCH model

Synchrotron SAXS data from solutions of Protein sex-lethal mutant with 10GS-linker in 10 mM KP, 50 mM NaCl, 10 mM DTT, pH 6 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Protein sex-lethal mutant (Sxl10GS)
Mol. type		Protein
Organism		Drosophila melanogaster
Olig. state		Monomer
Mon. MW		20.4 kDa

UniProt		P19339 (123-294)
Sequence		FASTA