Fuertes G,
Banterle N,
Ruff KM,
Chowdhury A,
Mercadante D,
Koehler C,
Kachala M,
Estrada Girona G,
Milles S,
Mishra A,
Onck PR,
Gräter F,
Esteban-Martín S,
Pappu RV,
Svergun DI,
Lemke EA,
Proc Natl Acad Sci U S A
114(31):E6342-E6351
(2017)
Europe PMC
SASDE83 – Labeled cold shock protein (CSP-Alexa488/Alexa594) with denaturant
Synchrotron SAXS
data from solutions of
Labeled cold shock protein (CSP-Alexa488/Alexa594) with denaturant
in
PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH 7.4
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 0.1 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 10.00 mg/ml was measured
at 23°C.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The protein contains a penultimate non-canonical amino acid p-acetylphenylalanine (207 Da) that is represented as U (selenocysteine, 168 Da) in the amino acid sequence for the entry. Therefore, the calculated MW from sequence (MW(expected)) must be adjusted accordingly (ca. 40 Da).
s, nm-1
