| MWexperimental | 64 | kDa |
| MWexpected | 47 | kDa |
| VPorod | 110 | nm3 |
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log I(s)
9.40×10-1
9.40×10-2
9.40×10-3
9.40×10-4
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s, nm-1
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Structural Insights into Bacteriophage GIL01 gp7 Inhibition of Host LexA Repressor.
Caveney NA, Pavlin A, Caballero G, Bahun M, Hodnik V, de Castro L, Fornelos N, Butala M, Strynadka NCJ, Structure 27(7):1094-1102.e4 (2019) Europe PMCSASDEB8 – Bacillus thuringiensis LexA repressor (Bt_LexA)
Bacillus thuringiensis LexA repressor|
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Fits and models
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SAXS data from solutions of Bacillus thuringiensis LexA repressor (Bt_LexA) in 20 mM HEPES, 300 mM NaCl, 10% glycerol, pH 8 were collected on a Rigaku BioSAXS-2000 instrument at the University of British Columbia (Vancouver, Canada) using a Hybrid Pixel Array Detector Rigaku HyOix-3000 detector at a wavelength of λ = 0.154 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 5 and 40 mg/ml were measured at 6°C. 12 successive 300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Sample detector distance = UNKNOWN |
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