A Metastable Contact and Structural Disorder in the Estrogen Receptor Transactivation Domain.

Peng Y, Cao S, Kiselar J, Xiao X, Du Z, Hsieh A, Ko S, Chen Y, Agrawal P, Zheng W, Shi W, Jiang W, Yang L, Chance MR, Surewicz WK, Buck M, Yang S, Structure 27(2):229-240.e4 (2019) Europe PMC

SASDEE2 – The N-terminal domain of estrogen receptor alpha

Estrogen receptor
MWexperimental 20 kDa
MWexpected 20 kDa
log I(s) 9.21×100 9.21×10-1 9.21×10-2 9.21×10-3
Estrogen receptor small angle scattering data  s, nm-1
ln I(s)
Estrogen receptor Guinier plot ln 9.21×100 Rg: 3 nm 0 (3 nm)-2 s2
(sRg)2I(s)/I(0)
Estrogen receptor Kratky plot 1.104 0 √3 sRg
p(r)
Estrogen receptor pair distance distribution function Rg: 3.1 nm 0 Dmax: 10 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Estrogen receptor CUSTOM IN-HOUSE model
log I(s)
 s, nm-1
Estrogen receptor CUSTOM IN-HOUSE model
log I(s)
 s, nm-1
Estrogen receptor CUSTOM IN-HOUSE model
Synchrotron SAXS data from solutions of the N-terminal domain of estrogen receptor alpha in 20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEP, pH 7.4 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source (NSLS-II; Brookhaven, NY, USA) using a Pilatus3 S 1M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.09 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.50 mg/ml was measured at 10°C. Three successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN

Tags: idp
Estrogen receptor (ER-NTD)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   20.2 kDa
 
UniProt   P03372 (1-184)
Sequence   FASTA