Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space.

Weinhäupl K, Lindau C, Hessel A, Wang Y, Schütze C, Jores T, Melchionda L, Schönfisch B, Kalbacher H, Bersch B, Rapaport D, Brennich M, Lindorff-Larsen K, Wiedemann N, Schanda P, Cell 175(5):1365-1379.e25 (2018) Europe PMC

SASDEF2 – Mitochondrial import inner membrane translocase complex TIM9·10

Mitochondrial import inner membrane translocase subunit TIM9
Mitochondrial import inner membrane translocase subunit TIM10
MWI(0) 50 kDa
MWexpected 61 kDa
VPorod 103 nm3
log I(s) 4.99×101 4.99×100 4.99×10-1 4.99×10-2
Mitochondrial import inner membrane translocase subunit TIM9 Mitochondrial import inner membrane translocase subunit TIM10 small angle scattering data  s, nm-1
ln I(s)
Mitochondrial import inner membrane translocase subunit TIM9 Mitochondrial import inner membrane translocase subunit TIM10 Guinier plot ln 5.00×101 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
Mitochondrial import inner membrane translocase subunit TIM9 Mitochondrial import inner membrane translocase subunit TIM10 Kratky plot 1.104 0 √3 sRg
p(r)
Mitochondrial import inner membrane translocase subunit TIM9 Mitochondrial import inner membrane translocase subunit TIM10 pair distance distribution function Rg: 2.7 nm 0 Dmax: 10 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Mitochondrial import inner membrane translocase subunit TIM9 Mitochondrial import inner membrane translocase subunit TIM10 CORAL model
log I(s)
 s, nm-1
Mitochondrial import inner membrane translocase subunit TIM9 Mitochondrial import inner membrane translocase subunit TIM10 DAMMIF model
log I(s)
 s, nm-1
Mitochondrial import inner membrane translocase subunit TIM9 Mitochondrial import inner membrane translocase subunit TIM10 GROMACS model
Synchrotron SAXS data from solutions of the mitochondrial import inner membrane translocase complex (TIM9·10) in 50mM Tris, 150mM NaCl, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5 mg/ml were measured at 20°C. One 20 second frame was collected per concentration. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Mitochondrial import inner membrane translocase subunit TIM9 (TIM9)
Mol. type   Protein
Organism   Saccharomyces cerevisiae
Olig. state   Trimer
Mon. MW   10.2 kDa
 
UniProt   O74700
Sequence   FASTA
 
Mitochondrial import inner membrane translocase subunit TIM10
Mol. type   Protein
Organism   Saccharomyces cerevisiae
Olig. state   Trimer
Mon. MW   10.2 kDa
 
UniProt   P87108
Sequence   FASTA
 
PDB ID   3DXR