Structure and Dynamics of a Promiscuous Xanthan Lyase from Paenibacillus nanensis and the Design of Variants with Increased Stability and Activity.

Jensen PF, Kadziola A, Comamala G, Segura DR, Anderson L, Poulsen JN, Rasmussen KK, Agarwal S, Sainathan RK, Monrad RN, Svendsen A, Nielsen JE, Lo Leggio L, Rand KD, Cell Chem Biol 26(2):191-202.e6 (2019) Europe PMC

SASDEG3 – Paenibacillus xanthan lyase (PXL) at 20 °C

Paenibacillus xanthan lyase
MWI(0) 121 kDa
MWexpected 113 kDa
VPorod 134 nm3
log I(s) 1.20×102 1.20×101 1.20×100 1.20×10-1
Paenibacillus xanthan lyase small angle scattering data  s, nm-1
ln I(s)
Paenibacillus xanthan lyase Guinier plot ln 1.21×102 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Paenibacillus xanthan lyase Kratky plot 1.104 0 √3 sRg
p(r)
Paenibacillus xanthan lyase pair distance distribution function Rg: 3.8 nm 0 Dmax: 13.8 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from a solution of Paenibacillus xanthan lyase (PXL) in 20 mM Tris pH 8.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.79 mg/ml was measured at 20°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Paenibacillus xanthan lyase (PXL)
Mol. type   Protein
Organism   Paenibacillus sp-62047
Olig. state   Monomer
Mon. MW   113 kDa
Sequence   FASTA