Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain.

Viet KK, Wagner A, Schwickert K, Hellwig N, Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA, Structure (2019) Europe PMC

SASDEH7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 4.5, 0.5 mM Calcium

Transient receptor potential channel mucolipin 2
MWexperimental 170 kDa
MWexpected 187 kDa
VPorod 280 nm3
log I(s) 8.69×101 8.69×100 8.69×10-1 8.69×10-2
Transient receptor potential channel mucolipin 2 small angle scattering data  s, nm-1
ln I(s)
Transient receptor potential channel mucolipin 2 Guinier plot ln 8.69×101 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Transient receptor potential channel mucolipin 2 Kratky plot 1.104 0 √3 sRg
p(r)
Transient receptor potential channel mucolipin 2 pair distance distribution function Rg: 3.7 nm 0 Dmax: 10 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Transient receptor potential channel mucolipin 2 CORAL model
log I(s)
 s, nm-1
Transient receptor potential channel mucolipin 2 DAMMIF model
Synchrotron SAXS data from solutions of Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain in 10 mM Hepes, pH 4.5, 150 mM NaCl, 0.5 mM CaCl2, were collected using size exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20°C using the following parameters: Column: Agilent BioSEC 3 bead size 300 μm, Ø 4.6 mm, length 300 mm; Flow rate: 0.3 mL/min; Total acquisition time: 20 min; Injection concentration: 10 mg/mL; Injection volume: 20 μL. The data obtained through the sample elution peak were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.

The experimental mass was determined via correlated volume.

Transient receptor potential channel mucolipin 2 (TRPML2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Octamer
Mon. MW   23.4 kDa
 
UniProt   Q8IZK6 (1-285)
Sequence   FASTA