Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes.

Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C, Kolbe M, J Mol Biol 431(19):3787-3803 (2019) Europe PMC

SASDEJ7 – The InvC-ATP synthase, surface presentation of antigens and oxygen-regulated invasion protein (ISO) complex

Surface presentation of antigens protein SpaO SpaO(SPOA2)
Surface presentation of antigens protein SpaO(SPOA1,2)
Oxygen-regulated invasion protein OrgB
ATP synthase InvC
MWexperimental 185 kDa
MWexpected 159 kDa
VPorod 302 nm3
log I(s) 4.17×103 4.17×102 4.17×101 4.17×100
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) Oxygen-regulated invasion protein OrgB ATP synthase InvC small angle scattering data  s, nm-1
ln I(s)
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) Oxygen-regulated invasion protein OrgB ATP synthase InvC Guinier plot ln 4.18×103 Rg: 6.0 nm 0 (6.0 nm)-2 s2
(sRg)2I(s)/I(0)
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) Oxygen-regulated invasion protein OrgB ATP synthase InvC Kratky plot 1.104 0 √3 sRg
p(r)
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) Oxygen-regulated invasion protein OrgB ATP synthase InvC pair distance distribution function Rg: 7.0 nm 0 Dmax: 22.7 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of ISO complex in 10 mM Tris-HCl, 50 mM NaCl, pH 8.0 were collected on the EMBL P12 beam line using size exclusion chromatography SAXS (SEC-SAXS) at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data obtained through the sample SEC-SAXS elution peak were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.

SEC-SAXS was performed at 20°C using the following parameters: Column type, XXXX; Flow rate: XXXX mL/min; Total acquisition time: 60 min (3600 x 1 s frames); Injection concentration: XXXX mg/mL; Injection volume: XXXX μL.

Surface presentation of antigens protein SpaO SpaO(SPOA2) (SpaOc)
Mol. type   Protein
Organism   Salmonella enterica subsp. enterica serovar Typhimurium
Olig. state   Dimer
Mon. MW   12.4 kDa
 
UniProt   P40699 (203-303)
Sequence   FASTA
 
Surface presentation of antigens protein SpaO(SPOA1,2) (SpaO)
Mol. type   Protein
Organism   Salmonella enterica subsp. enterica serovar Typhimurium
Olig. state   Monomer
Mon. MW   33.8 kDa
 
UniProt   P40699 (1-303)
Sequence   FASTA
 
Oxygen-regulated invasion protein OrgB (OrgB)
Mol. type   Protein
Organism   Salmonella enterica subsp. enterica serovar Typhimurium
Olig. state   Dimer
Mon. MW   26.5 kDa
 
UniProt   P0CL45 (1-226)
Sequence   FASTA
 
ATP synthase InvC (InvC)
Mol. type   Protein
Organism   Salmonella enterica subsp. enterica serovar Typhimurium
Olig. state   Monomer
Mon. MW   47.6 kDa
 
UniProt   E8XL22 (1-431)
Sequence   FASTA