Engineered variants provide new insight into the structural properties important for activity of the highly dynamic, trimeric protein disulfide isomerase ScsC from Proteus mirabilis.

Furlong EJ, Kurth F, Premkumar L, Whitten AE, Martin JL, Acta Crystallogr D Struct Biol 75(Pt 3):296-307 (2019) Europe PMC

SASDEK4 – C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein (ScsC)

C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein
MWI(0) 11 kDa
MWexpected 20 kDa
VPorod 22200 nm3
log I(s) 4.05×10-3 4.05×10-4 4.05×10-5 4.05×10-6
C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein small angle scattering data  s, nm-1
ln I(s)
C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein Guinier plot ln 4.06×10-3 Rg: 1.7 nm 0 (1.7 nm)-2 s2
(sRg)2I(s)/I(0)
C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein Kratky plot 1.104 0 √3 sRg
p(r)
C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein pair distance distribution function Rg: 1.7 nm 0 Dmax: 5.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein DAMFILT model
log I(s)
 s, nm-1
C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein PDB (PROTEIN DATA BANK) model
X-ray synchrotron radiation scattering data from solutions of the C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein (ScsC) protein from Proteus mirabilis in 10 mM HEPES 150mM NaCl, pH 7.4 were collected on the SAXS/WAXS beam line of the Australian Synchrotron (Melbourne, Australia) using a 2D Photon counting Pilatus 1M-W pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Thirty eight successive 1 second frames were collected across solute concentrations of 0.1-10.0 mg/ml at a sample temperature of 10°C. The SAXS data displayed this entry was derived from a 0.50 mg/ml sample. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of the solvent-blank was subtracted.

C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein (PmScsCΔN)
Mol. type   Protein
Organism   Proteus mirabilis
Olig. state   Monomer
Mon. MW   20.2 kDa
 
UniProt   A0A1Z1SYD5 (64-243)
Sequence   FASTA
 
PDB ID   4YX8