Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes.

Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C, Kolbe M, J Mol Biol 431(19):3787-3803 (2019) Europe PMC

SASDEK7 – SpaO C-terminus

Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus
MWexperimental 20 kDa
MWexpected 19 kDa
log I(s) 2.94×103 2.94×102 2.94×101 2.94×100
Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus small angle scattering data  s, nm-1
ln I(s)
Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus Guinier plot ln 2.95×103 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus Kratky plot 1.104 0 √3 sRg
p(r)
Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus pair distance distribution function Rg: 2.1 nm 0 Dmax: 7.2 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of SpaO C-terminus in 20 mM HEPES pH 7.5, 150 mM NaCl, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). 3600 successive 1 second frames were collected at 20°C using size-exclusion chromatography SAXS. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

SEC-SAXS was performed at 20°C using the following parameters: Column type, XXXX; Flow rate: XXXX mL/min; Total acquisition time: 60 min (3600 x 1 s frames); Injection concentration: XXXX mg/mL; Injection volume: XXXX μL.

Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus (SpaO C-terminus)
Mol. type   Protein
Organism   Salmonella enterica subsp. enterica serovar Typhimurium
Olig. state   Monomer
Mon. MW   19 kDa
 
UniProt   P40699 (140-297)
Sequence   FASTA