SASDEL7 – Human glycine decarboxylase

Glycine decarboxylase

MW^experimental	190	kDa
MW^expected	219	kDa
V^Porod	304	nm³

log I(s) 1.67×10² 1.67×10¹ 1.67×10⁰ 1.67×10^-1

Glycine decarboxylase small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 1.68×10² R_g: 3.9 nm 0 (3.9 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

Data validation

Fits and models

log I(s)

s, nm^-1

Glycine decarboxylase PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of human glycine decarboxylase in 10 mM TRIS pH 7.5, 100 mM NaCl, 1 mM DTT, were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 20°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Glycine decarboxylase (GLDC)
Mol. type		Protein
Organism		Homo sapiens
Olig. state		Dimer
Mon. MW		109.4 kDa

UniProt		P23378 (45-1020)
Sequence		FASTA

PDB ID		6I33