| MWI(0) | 75 | kDa |
| MWexpected | 75 | kDa |
|
log I(s)
2.72×10-1
2.72×10-2
2.72×10-3
2.72×10-4
|
s, nm-1
|
Migration of Type III Secretion System Transcriptional Regulators Links Gene Expression to Secretion.
Charova SN, Gazi AD, Mylonas E, Pozidis C, Sabarit B, Anagnostou D, Psatha K, Aivaliotis M, Beuzon CR, Panopoulos NJ, Kokkinidis M, MBio 9(4) (2018) Europe PMCSASDEM4 – HrpG/HrpV/HrpJ gatekeeper complex from Erwinia amylovora
Type III secretion protein HrpGType III secretion protein HrpV
Hypersensitivity response secretion protein HrpJ
|
|
|
Fits and models
|
|
|
Synchrotron SAXS
data from solutions of
HrpG/HrpV/HrpJ gatekeeper complex from Erwinia amylovora
in
20mM Tris-Cl, pH 8.0, 100mM NaCl, 2mM DTT, 0.5mM EDTA, pH 8
were collected
on the
SWING beam line
at the SOLEIL storage ring
(Saint-Aubin, France)
using a AVIEX PCCD170170 detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.1033 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 1.50 mg/ml was measured
at 15°C.
30 successive
0.500 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
|
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||